Advances in Fruit Crop Breeding: Conventional and Molecular Approaches

Shashi Ranjan Pratap Singh

Bhola Paswan Shastri Agricultural College, Purnea (BAU, Sabour, Bhagalpur), Bihar, India.

Jai Prakash Prasad *

Bhola Paswan Shastri Agricultural College, Purnea (BAU, Sabour, Bhagalpur), Bihar, India.

*Author to whom correspondence should be addressed.


Abstract

Fruit crop breeding has transformed from traditional selection to advanced molecular techniques, significantly enhancing productivity and climate resilience. Conventional methods—including mass selection, pure line selection, hybridization, and mutation breeding—laid the foundation for varietal innovation. Hybridization, specifically, has been instrumental in pyramiding desirable traits and exploiting heterosis to create superior F1 hybrids in species like papaya and passion fruit. The integration of molecular tools like Marker-Assisted Selection (MAS), Genomic Selection (GS), and CRISPR/Cas9 gene editing has drastically reduced breeding cycles. MAS allows for early-stage selection of traits such as disease resistance and fruit quality, while GS predicts breeding values across the entire genome, overcoming the limitations of traditional phenotyping. CRISPR/Cas9 offers unprecedented precision for targeted gene knockouts or sequence replacements, addressing challenges like shelf-life extension and nutritional fortification. Indian research institutions, including IIHR and CISH, have contributed significantly by developing improved varieties of mango (e.g., Amrapali), banana, and citrus tailored to local agro-climatic conditions. Despite these successes, implementation is hindered by insufficient funding, poor infrastructure, regulatory complexities, and a shortage of skilled personnel. Future advancements lie in speed breeding, artificial intelligence-based predictive modeling, and multi-omics integration. Modernizing infrastructure and streamlining regulatory frameworks are essential for India to guarantee food security and economic sustainability through sustainable fruit crop improvement.

Keywords: Fruit breeding, molecular markers, CRISPR, genomic selection, Indian varieties, crop improvement


How to Cite

Singh, Shashi Ranjan Pratap, and Jai Prakash Prasad. 2026. “Advances in Fruit Crop Breeding: Conventional and Molecular Approaches”. Archives of Current Research International 26 (4):68-85. https://doi.org/10.9734/acri/2026/v26i41805.

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