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Peach (Prunus persica L. Batsch) is an important temperate fruit crop valued for its nutritional and economic importance. Bio-stimulants such as seaweed extract are increasingly used in horticulture to enhance plant growth, yield, and fruit quality by improving nutrient uptake and stress tolerance. Therefore, this study evaluates the effect of seaweed extract on growth, yield, and fruit quality of peach cv. Shan-e-Punjab under field conditions. A field experiment was conducted at Orchard 1 in the Department of Fruit Science, Guru Kashi University, Talwandi Sabo, Bathinda, Punjab, during the year 2024-2025. A Randomized Block Design (RBD) with three replications and ten treatments was used in the investigation. The purpose of this research is to investigate the effect of SWE because insufficient data exist on how these specific application methods differently affect vegetative growth, flowering, and fruiting behavior, and there is a limited understanding of how pre-harvest seaweed extract applications influence post-harvest fruit quality, shelf-life, and final mineral composition. The study consists of four different concentrations of SWE: 1000 ppm, 1500 ppm, and 2000 ppm were applied at three different stages pink bud stage, petal fall stage and pit hardening stage. The results within this study demonstrated that treatments with SWE greatly enhanced plant development, production, fruit quality, and both the macro and micronutrient concentration of leaves. The concentration (2000 ppm) of seaweed extract had the largest plant volume, fruit production, fruit set, fruit retention, area of leaves, size, along with weight of fruits, fruit firmness, total sugars, maximum total soluble solids, and leaf nutrient parameters.